Bioassay of cytokinin
WebCytokinin is synthesized in the roots from where they are transported to shoots by xylem tissues. Biosynthesis of purine type of cytokinin is more clearly known than of other types. Various components of purine are derived from several small molecules including formyltetrahydro folate, aspartate, CO 2, ... WebAbstract A rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after seed germination. ... naturally occurring substances without cytokinin activity, induce small growth increments in the assay. Cytokinin structure-activity relationships ...
Bioassay of cytokinin
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WebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss and for a wide range of natural and synthetic cytokinins, to date only substituted adenine cytokinins have been examined in detail. This paper shows that at least some ... WebJul 1, 1992 · The bioassay is based on the expression in transformed tobacco ( Nicotiana tabacum) mesophyll protoplasts of a chimeric gene, consisting of the upstream …
WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been … Web1-1. Introduction. We performed quantitative analysis for cytokinin using cotyledons (seed sprouts) of cucumber (Cucumis sativus L. cv.). We proposed to make E. coli produce cytokinin and needed to establish experimental system for quantitative analysis for cytokinin. Cucumber cotyledons are frequently used as samples for a simple and rapid …
WebA bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: … Weband the soybean callus bioassay. The highest con-centration of cytokinin was DHZR at 35.6 ug 1-1 followed by iP 15.9 jig 1-1. The cytokinin with the lowest concentration was tZ at 0.7 pg 1-1. The total cytokinin concentration in Seasol was 0.115 mg 1-1 (Tay et al., 1985). Zeatin-O-glucoside (ZOG) and
WebHer primary areas of investigation include Botany, Cytokinin, Food science, Horticulture and Shoot. Her study in Botany is interdisciplinary in nature, drawing from both Micropropagation and Tissue culture. Her work in the fields of Zeatin overlaps with other areas such as Seedling, Germination, Bioassay and Callus.
WebThe name cytokinin is because of its specific effects on cytokinesis. Kinetin and certain other substances show kinetin-like activity, collectively called cytokinins. ... and a three times higher CK content as judged from a bioassay of root exudate. Since cytokinins promote cell division, it is interesting that they should show binding affinity ... ircc advanced analyticsWebshortcoming of these is that the cytokinin nucleotides, which are considered to be of major significance in cytokinin biosynthesis and metabolism [7] were not included. This paper reports on the response of 28 authentic cytokinin derivatives, including nucleotides, in the soybean callus bioassay, which is widely used in cytokinin research. 2. ircc afghan refugee programWebMar 1, 1974 · Cucumber cotyledon expanSIon in dark can therefore serve as a bioassay system for cytokinins. A comparison of the activity of different concentrations of kinetin and benzylaminopurine on cotyledon expansion in dark is shown in table 6 and figs. 3 to 5. It will be seen that benzylaminopurine is more active than kinetin in cucumber cotyledons ... order chp 180 formsWebThe test can measure cytokinin concentration between 0.001-10 mg/litre. It takes 3-5 weeks. 2. Retardation of Leaf Senescence: It is a rapid bioassay technique. Leaf discs … ircc acknowledgement letterWebkinin bioassay. Cytokinin-like activity was determined by the radish cotyledon assay. The radish cotyledon bioassay. Radish seeds, Raphanus sativus L. cv. Okura Daikon, were germinated in darkness at 27°C on wet filter paper in plastic boxes. After about 3 days, larger cotyledons were excised from each ircc afghanistan programWebFeb 1, 1998 · The purpose of these protocols is to provide practical stepwise methods for the bioassay of cytokines using cytokine responsive cell lines. They include tables of the … ircc afghan refugeesWebA rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after … order chris brothers pepperoni online