Webb1 apr. 2002 · We also studied whether green fluorescent protein (GFP) and drFP583 could be detected simultaneously from a single bacterial cell. Methods: Plasmids pDsRed and … Webb12 apr. 2024 · c Relative mean fluorescence intensity (MFI) of GFP and CD69 expression from b. Data were normalized to PMA/Ionomycin treatment and were presented as …
Influence of structural dynamics on cell uptake investigated with ...
Webb1 apr. 2002 · To produce enhanced GFP and drFP583 simultaneously, plasmids pG9R and pG19R were constructed. These encode tandem fusions of enhanced GFP and drFP583 to ensure similar production levels for both proteins. Results: Bacteria producing enhanced GFP and drFP583 were found to be brightly green and red fluorescent, respectively. Webb1 sep. 2024 · All MFI measurements in transfected cells are blank-corrected with respective MFI values from the transfected cells that are used as negative control (e.g. MFI FITC in transfected minus MFI FITC values in cells transfected with unlabeled plasmid). emb category c projects
NFAT-controlled expression of GFP permits visualization and …
Webb17 aug. 2024 · Thus, here’s a short set of instructions for using it to do a basic process, such as determining what percentage of live cells are also GFP positive. Obviously, if you don’t have FlowJo yet, then download it … WebbMean GFP intensity in PBMU vs. CBMU declined rapidly (152 MFI 6 70 MFI vs. 112 MFI 6 64 MFI after 15 min and 29 MFI 6 6MFIvs.26MFI6 3 MFI after 75 min; P <0.05 vs. 15 min; Fig. 2B). After 105 min, mean GFP intensity reached a basallevelofabout 20MFI. DISCUSSION Using a flow cytometric assay that combines functional Webb22 okt. 2013 · Interestingly, GFP expression in U87 cells stably transduced by RGD4C/AAVP-Grp78-GFP increased at the level of MFI only, upon TSA treatment, with no effect on GFP positive cells (Figure 2B). Next, we investigated additional HDAC inhibitors such as suberoylanilide hydroxamic acid (SAHA), which is structurally similar to TSA, … ford transit temperature sensor location