WebJan 17, 2024 · Using a serial dilution, describe how you would prepare 10 mL of a 1%, 0.1% and 0.01% solution of NaOH. The stock solution of NaOH is 10%. Draw diagram as part of your description. Using the standard curve below, calculate the concentration of an unknown solution if its absorbance is 0.55. Figure 3. WebLSBio

A dataset of global ocean alkaline phosphatase activity

WebStandard curve was prepared using pnitrophenol. 600 μl substrate buffer (3 mM pnitrophenylphosphate, 5mM MgCl2, 0.1% Triton X-100 and 50 mM Glycine-NaOH buffer, pH 10.4) was added to 1.5 mL... Webcompound at 405nm using a standard curve as in experiment 1). Following the determination of E you will calculate reaction rate. Influence of pH on phosphatase … city of king public utilities

Experiment 7 (Lab Period 8) Quantitative Determination of …

WebLab 1 Objective: construct PNP standard curve and use this to determine [ ] of a PNP dilution micropipette Volume range P20 2.00-20.00 µl P200 10.0-200 µl P1000 200-1000 µl 1 µl= 10^-6 L 1 mL= 10^-3 L Prep dilutions of PNP → measure A400 → prepare standard curve → prepare assigned dilution of PNP → measure A400 of dilution → ... WebPNPP has apparent Km values for protein phosphatases in the range of 0.5 -10 mM (2-5). One unit of the protein phosphatase activity is defined as the amount of enzyme that hydrolyzes 1 nanomole of PNPP in one minute at 30°C in a total reaction volume of 50 μl … WebJan 15, 2024 · The UV–vis absorption spectra were obtained using the multimode plate reader (mPlex Pro, Tecan, Instruments), and the molarity (in mM) was calculated using the standard curves of pNPP (max = 310 nm) and pNP (max = 405 nm). The following equation is used to determine the yield (%) of pNP: Y i e l d o f p N P = X t X 0 × 100 % city of kingman weather